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From 2010.igem.org

Contents 1 PCR/pBS1C3 1.1 member 1.2 materials,Procedure 2 Agarose Gels Electro-phoresis 2.1 member 2.2 Materials,Procedure 3 Experiment:Make Agarose Gels Electro-phoresis 3.1 Member 3.2 Materials and Procedure 4 Medium making 4.1 member 4.2 materials and procedure

PCR/pBS1C3

member

naoto and bambi75

materials,Procedure

Same on August 23th.

• 95℃3min
• 96℃1min
• 51℃1min
• 72℃3min
• 10℃∞

（96℃～72℃/30cycle）

Agarose Gels Electro-phoresis

member

Same above

Materials,Procedure

Same protocol on August 16th. PCR Product of insert is pBS1C3

Experiment:Make Agarose Gels Electro-phoresis

Member

Same above

Materials and Procedure

Same protocol on August 16th.

Medium making

member

Same above

materials and procedure

Same protocol on August 10th.