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Talk:Team:IvyTech-South Bend/8 October 2010
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Revision as of 20:34, 17 October 2010 by Rchamberlin (Talk | contribs)
10/8/10
Making 4 1L bottlse of LB-Lennox Broth
2- with Agar 2- without
I placed between 10.035 - 10.005 grams into each of the 4 bottles, then added 500 ml 18 ohm H2O into each bottle
Placed into the microwave on beverage setting 8oz.
after first time removed the 2 -just broth
heated the 2- with Agar again at the same setting.
Added to 2- with Agar - 7.002 grams to each then placed into the autoclave on liquid setting/ 121 C for 15 min.
After done , cool to room temp label and place into fridge until needed.
