Microscopy

Details on microscope

Figure 1: Picture of the automatized microscope.

A microscope with motorized x, y and z control, a motorized shutter and a 60× lens is used with appropriate fluorescence filters for the fluorescence signals. Light-emitting diode arrays are installed as light sources for red light (660 nm) and far-red light (748 nm) pulses.

Details on the Flow Channel

Figure 2: The mold for the channel. Has to be cleaned after casting.	Figure 3: The casted raw channel with a cover slip and the razor plate needed for preparing the channel.	Figure 4: The assembled channel, yet not bonded.
Figure 5: Bonding the channel.	Figure 6: The ready to use channel under the microscope.