September, 08th

Production Team

David Caballero. We performed the second overlapping PCR reaction of the SDM with the former and purified PCR products. We carried out two strategies: in one PCR reaction we used the original concentration and in another we used a 1/10 dilution of it.

Products of these PCR reactions were analyzed in 0,8% gel electrophoresis and it was shown that there were not amplification.

Assembly Team

Transformation of E. coli DH5-alfa with the products of ligations, and we spread in selective plates.