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Team:Aberdeen Scotland/Protocols
From 2010.igem.org
University of Aberdeen - ayeSwitch
iGEM 2010
Protocols
For our iGEM2010 project, we have used a variety of techniques such as -
Restriction digest of DNA plasmids
Gel electropheresis of DNA products to check for correct length and quantity of DNA present
Selective culture of E-coli and Yeast in liquid and Agar Medium (LB and SD)
PCR Amplification
Transformation of shuttle vectors into yeast
DNA extraction for E-coli by Spin Mini-Prep
Microscopy, Fluorimetry and FACS
