Team:WITS-South Africa/Machine Design

Intermediate Machine 2 (Figure 1) Purpose: a) To characterise the strength of the induction of the LacI/AraC promoter by measuring fluorescence after IPTG is added b) To show that the PlcR promoter is activated in L. gasseri by measuring fluorescence after the addition of exogenous PlcR and PapR proteins

Fig1. The proposed intermediate Machine 2

Final Machine 1 (Figure 2) Purpose: a) To act as the ‘Detector’ Machine within the population and produce the quorum signalling peptide in response to an input signal

Fig 2. The proposed Final Machine 1

Final Machine 2 (Figure 3) Purpose a) To act as the ‘Reporter’ Machine within the population and respond to infection as previously described

Fig 3. The proposed Final Machine 2