Team:Calgary/18 June 2010

From 2010.igem.org

Friday June 18, 2010

Gel electrophoresis of Jeremy's construction of B0015-R0040 and Himika's construct of E1010-B0015

Emily: Today I did a restriction digest of the arabinose promter (I0500) using all combinations of restriction enzymes. The sequencing of this part came back very strange, so I want to verify the presence of all of the Biobrick cut sites within the part. Today I also started a colony PCR using the BBK Construction primers of my I0500-B0034 construct that I constructed yesterday.

Dev, Jeremy, and Chris: Today, Jeremy did gel electrophoresis of different colonies from his construction of B0015-R0040 and Himika's construct of E1010-B0015 which is shown in the photo on the right. The bands observed were much clearer than those of the gel done yesterday. We had a group meeting in the afternoon to discuss with Dr. Logan and Deirdre about the progress during the week. Dr. Logan pointed out a cheaper method to verify whether the DNA was present by searching for restriction sites within the DNA, digesting with them, and checking the size of the parts with a gel electrophoresis. Chris's plates from yesterday of J13002-E0032 and pSB1K2-J23032 showed no growth once again. They will be attempted again next week with the vector and insert reversed.