Talk:Team:Groningen

From 2010.igem.org

current brainstorming (12 march 2010):

-Food: loose weight consumes fat and chlorestorial

We could design probiotic bacteria (Like that useless Actimel crap but useful!! Imagine that!!1!) which are destined to waste a certain amount of calories. They would be eaten added to yoghurt or the like (In contrast to Actimel we could even make it tasty). So lets say for some reason you feel like not becoming a diabetic or a heart attack victim, you just compensate all the extra calories by munching our genetically modified never tested on animals or the like bacteria which we only have a vague idea of what they might do and TARAAA! Technology beats nature… again. The easiest would be to get the bacteria to produce something that can’t be digested in the intestines like fat or even better, a substance that smells good (for obvious reasons) After a while the indigenous bacteria will outcompete our sissy lab bacteria and everything is back to normal –Ramon Sieber

-Biodegradable fabric: good & green

-Fertilizing Gounds: cactus, nitrogen fixing compatible to all plants

-Bacteria -> Colon Cancer

First of all you need to think of what cells youd want to detect: stem cells could be interesting Identification of Gastric Cancer Stem Cells Using the Cell Surface Marker CD44-Takaishi, but of course a stem cell is just that, there is no cancer yet. (also: Phenotypic characterization of human colorectal cancer stem cells - Dalerba) A lot of different markers: http://www.antibodybeyond.com/reviews/tumor-markers/colorectal-cancer-marker.htm, FAS overexpression could be a good one too. http://www.tc-cancer.com/tumormarkers.html

Als interesting: The effect of certain gut bacteria on the development and maybe progression of colon cancer: Probiotics. These characterisation might help choose a test model. http://www.google.nl/search?hl=nl&q=bacteria+apoptosis+cancer+cells+colon&meta=&aq=f&aqi=&aql=&oq=&gs_rfai= E.coli is able to do this via BMP2 for example and human proteins that have alike functions can ofcourse all be produced in E.Coli.

Dangers: Infections

-Laundry machine -> encymes producing bacteria for washing machines

-Sponge - ISOLATION

-biological Anti freeze so it can detect temperature change and unfreeze for example the car window.

-Bacteria to reduce air pollution

-Use bacteria as a memory/harddisk ->Computer by Bacteria

-Oil spill responce - Baceria cleaning it up.

After oil spills the oil which is not removed mechanically is mostly degraded by bacteria (bioremediation) so far only adding fertilizer seems to help, the addition of living bacteria has mostly not shown any effect. I can think of three ways to use bacteria in order to improve bioremediation, 1: Nitrogen fixing bacteria could be added instead of fertilizer, Nitrogen fixing bacteria already exist, but I don’t know if they can life in oily environements. 2. Bacteria which can metabolize the compounds which so far can not be degraded by bacteria 3. Bacteria which are actually better at degrading oil than existing ones ( I have however no clue how that could be achived) 1&3 would enhance speed, 2 would improve endpiont which has so far not been attempted (at least not from what I have read) -Marine microorganisms make a meal out of oil, nature reviews 2006 -Field evaluation of marine oil spill bioremediation, microbial reviews 1996 (Very long but also very complete review about the topic) -Ramon Sieber

-Detecting clusters of bacteria

-Synthetic biomolecules on synthetic biomolecules

-Magnetic bacteria-two bacteria spin around each other and create a magnetic field,which we can measure(*)

-bacteria changing the taste of yogurt

-making a bacterial community( to mimic the interaction between bacteia in real world)

-biocamera( there are bacteria which sensitivi different kind of signal, when they are exposed, they are giving a shape on the medium (take pic!)

-UV detected bacteria

-bacteria that has a capable of altering one type of aa to another or producing essential amino acid

-molecular fishing rod

Using the Biotin – Avidin connection bacterias could maybee ( I don’t know how strong that connection really is, but it is the strongest at hand) be bound to a surface or the like. The bacteria could express biotin or avidin to the surface (the bricks exist) at a certain metabolic state, on command, as long as they are allive, when you flash them with light or whatever… and then be fished out of the solution with a device coated with Biotin or avidin. -Ramon Sieber

-biofilm color

-Detection of movement

A system consisting of two organisms, a) Bacteria and b) a creature abel to move over solid surface (Amoeba, nematode, snail…) . The bacteria would cover the ground (agar plate) completely and would detect the organism moving over them, either by a chemical which this organism releases or by pressure, depending on size of the moving agent pressure might not work, and start to produce a fluorescent or colour signal hence leaving a mark of the organisms way (I know snails do that anyway but…) Using chemotaxis the organisms could be sent through a maze or be steered in order to gain world domination.

- The tron version of movement detection

In addition to a colour or fluorescence marker the bacteria also produce a repellent for the used organism. This will hinder the organism of crossing its own path or the path of another “Player”. Which basically is what Tron does. For all sorry souls who have never played it: http://gamepuma.com/action-games/Tron.html -Ramon Sieber

-restore gulf stream pump once it breakes down

-anti-rust bacteria

-anti-anxiety bacteria

-anti-acidification bacteria for ocean

-maleria repelling bacteria

-smelling (coffee) of circadian rythm bacteria

Cells which react to a long period of darkness (night) followed by light (the sensors for light exist so this should be possible) produce either smell of coffe or caffein (your blanket is a biofilm so you absorbe it) to assure a gentle awakening –Ramon Sieber

-timer bacteria

The bacterio'clock is not a new idea. Godfathers of synthetic oscilator systems: Elowitz and Leibler and their repressilator system. What would be interesting is to try and make a system that responds to certain cue's or produces something like an aroma or medicin. Or a brand new system, to submit awesome new biobricks and systems. A circadian rythm or a rythm to adjust bacterial or yeast behaviour. Previous iGEM projects: Paris08, Shanghai09, Michigan 2008 (the sequencilator or something like that). Martha Merrow was enthousiastic about a possible project and offered advice. Also interesting: oscillating systems. http://www.nature.com/nature/journal/v463/n7279/full/nature08753.html - Peter Roemers

-spore eating bacteria

-detect bad milk bacteria

-biofilm insulating bacteria (bacteria cover the building)

-two component gene made by bacteria stolling factor

-vitamine rich food , nice tasting food

IGEM Meeting(15.03.2010)

-anti-hangover bacteria

-quit smoking bacteria

-plactic degrading bacteria(microplastic degrading bacteria): We've been beaten by puberty: http://www.wired.com/wiredscience/2008/05/teen-decomposes/,

-caffeine producing bacteria

-gas transporter bacteria

-filter bacteria

-bacteria against the skin disease

-bioglue

-melanin production stimulating baceria

-carbon tubes producing bacteria

-flying and ozone producing bacteria

-Alcohol content controll by apoptosis

Right now alcohol content is controlled either by sugar availability, cytotoxicity, or manual killing of the yeast at a certain alcohol level (the idea could be applied to other fermentation products as well). It would be usefull to have a system in which you could decide beforehand to which degree the yeast will grow, this could be done by getting the yeast to recognize a certain ratio between alcohol and a second substance and kill themselves once that ratio is reached. By dosing that second substance it could be determined when the yeast starts killing itself and hence the final alcohol level. - Ramon Sieber

-seismo detecting bacteria(sound detecting bacteria)

-keyboard( pressure sensitive bacteria)

-glitter bacteria

-sparkling bacteria

-caffeine biobrick

-cake degrading bacteria

- Anti-body binding bacteria: for numerous functions, targeted that is

@Home ideas (Maarten):

-Make bacteria produce a certain compound (like a pigment) which you'll want to apply to a surface of some sort. Let them first produce it cytosolic and then let them secrete it by a light sensitive channel. These channels are the MscL (Mechanoselective channels of Large conductance) and have been modified to be light and pH sensitive in stead (A. Koçer, 2007). I think we can use this mechanism for several for our ideas, e.g. biofilm paint and the detection of cancerous cells, which have a lower pH when compared to normal cells. The bacteria (for cancer in the GI tract) could then secrete some sort of detectable marker. Lipid bodies could be used for detection in the rest of the body.

-Bacteria that import vitamins or minerals and inject them into plant cells to make them more nutritional.