"

From 2010.igem.org

Week12 Summary

We began assembling LacP-RBS-CHS3. We defrosted our -20 and in the process reorganized the -20 and threw out unnecessary DNA.

---

8/30/10

Screened MAGE culture for knockouts.

---

8/31/10

Attempted to assemble LacP-RBS-CHS3 --> accidentally cut CHS3 with P (contains a P site)

Reorganized the racks in the -20.

Threw out unnecessary/old DNA from our -20.

Moved all of our racks from our -20 to another -20 in order to defrost our -20.

tqsA Knockouts FOUND (?) in MAGE culture upon screening.

---

9/1/10

Ragan left for home today and left MAGE in our hands. He asked us to test his tsqA knockouts to determine if they were in fact true knockouts.

9/2/10

---

9/3/10

Reorganized -20 DNA stock. Created racks for individual teammates to stay organized once the school year starts.

---