Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14

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Contents

2010/10/14

1:Electrophoresis

<Member>
nito

<Sample>

  • 1-2-7
  • 1-2
  • 10-2-7
  • 9-7
  • 4-5


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-10-14 14hr 29min.JPG

2：DNA extraction

＜Member＞
nito

＜Sample＞

  • 1-2-7
  • 10-2-7
  • 9-7

＜Protocol＞
See Protocol 4

3：DNA Ligation

＜Member＞
nito

＜Sample＞

  • 4-5
  • 1-2-3
  • 10-2-3
  • 10-2-9
  • 7-8-10

(after digestion)

＜Protocol＞
See Protocol 10

4：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

  • plasmid

・Digest enzyme

  • XbaⅠ
  • SpeⅠ

＜Protocol＞
See Prptpcpl 9

5:PCR

<Member>
nito

<Sample>

  • vector
  • 4-5
  • 1-2-3
  • 10-2-3
  • 7-8-10
  • 10-2-9


<Protocol>
See Protocol 1

6：DNA extraction from gels

＜Member＞
nito

＜Sample＞

  • 9-7

(After electrophoresis)


＜Protocol＞
SeeProtocol 4

7：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

  • 1-2
  • 1-2-7
  • 10-2-7

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • XbaⅠ
  • SpeⅠ

＜Protocol＞
See Prptpcpl 9

8:PCR

<Member>
nito

<Sample>

  • 9
  • 9-7


<Protocol>
See Protocol 1

9：DNA extraction from gels

＜Member＞
nito

＜Sample＞

  • 9-7

(After electrophoresis)


＜Protocol＞
SeeProtocol 4

10:Electrophoresis

<Member>
nito

<Sample>

  • vector
  • 9-7
  • 4-5


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-10-14 22hr 56min.JPG

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