Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14
From 2010.igem.org
Contents |
2010/10/14
1:Electrophoresis
<Member>
nito
<Sample>
- 1-2-7
- 1-2
- 10-2-7
- 9-7
- 4-5
<Protocol>
See Protocol 8
2:DNA extraction
<Member>
nito
<Sample>
- 1-2-7
- 10-2-7
- 9-7
<Protocol>
See Protocol 4
3:DNA Ligation
<Member>
nito
<Sample>
- 4-5
- 1-2-3
- 10-2-3
- 10-2-9
- 7-8-10
(after digestion)
<Protocol>
See Protocol 10
4:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
- plasmid
・Digest enzyme
- XbaⅠ
- SpeⅠ
<Protocol>
See Prptpcpl 9
1:PCR
<Member>
mio
<Sample>
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
<Protocol>
See Protocol 1
7:DNA extraction from gels
<Member>
<Sample>
- 1+2(8/31)
- 4+5(8/31)
(After electrophoresis)
<Protocol>
SeeProtocol 4
7:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
- 1
- 2
- 3
- 4
- 6
- 10
・Digest enzyme
- AvrⅡ
- NheⅠ
<Protocol>
See Prptpcpl 9
1:PCR
<Member>
mio
<Sample>
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
<Protocol>
See Protocol 1
7:DNA extraction from gels
<Member>
<Sample>
- 1+2(8/31)
- 4+5(8/31)
(After electrophoresis)
<Protocol>
SeeProtocol 4
3:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8