Team:TU Delft/Project/alkane-degradation/results

From 2010.igem.org

Figure 1 – Schematic description of the alkane degradation pathway with the corresponding genes.

Alkb2 Cluster

LadA

ADH

ADH was tested with Dodecanal (C12)

This yielded the following results:

Figure 1 – The results of a resting cell assay done on biobrick number k398018.

This shows two things. That E.Coli is partly able to degrade Dodecanal on it's own. And that this activity is greatly increased (38.4%) with the addition of ADH. It is also clear that eventhough ADH is from a thermophilic organism it loses most of it's activity after heating. (Although this activity could also be attributed to the host organism lacking the proper post translational modifications.)

ALDH