Team:Harvard/fences/parts

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parts and primers

Biobricks

Protein inhibitor of BarnaseLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter
NameDescriptionResistanceSequence
Barnase Fatal gene for the genetic fence chloramphenicol
BarstarchloramphenicolnptII
LacINchloramphenicol
V4 TEXT INSERT TEXT HERE chloramphenicolnptII
V5 TEXT INSERT TEXT HERE chloramphenicolnptII


Primers

NameDescriptionSequence
Arp2mutForwardPoint mutagenesis to remove restriction enzyme site5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'
Arp2mutReversePoint mutagenesis to remove restriction enzyme site5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'
Exp2MutForwardPoint mutagenesis to remove restriction enzyme site5'-CCT ACA CAC GGA ACT CCT GGA AAT TAA GAC CG-3'
Exp2mutReversePoint mutagenesis to remove restriction enzyme site5'-CGG TCT TAA TTT CCA GGA ACT CCG TGT GTA GG-3'
Bam.Gal4.FwdAttaching BamH1 sites to Gal4 in preparation for yeast vector ligation5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'
HmRXR.Hind.RevAttaching HindIII sites to HmRXR in preparation for yeast vector ligation5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'
LcRXR.Hind.RevAttaching HindIII sites to LcRXR in preparation for yeast vector ligation5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3'
VP16.Hind.FwdAttaching HindIII sites to VP16 in preparation for yeast vector ligation5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3'
VP16.RevReverse primer for VP16.Hind.Fwd PCR - no modification5'-CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3'
NLS.serine.BB.FwdOligo to anneal NLS, with one bp difference to avoid self-complementarity 5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'
NLS.serine.BB.RevComplement to NLS.serine.BB.Fwd. for NLS annealing5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3'
Fwd.BB.AtEXP2for amplification/extraction of an arabidopsis germination-specific promoter5'-CCT TTC TAG ACC TGG TGT GGT TTC TTT G-3'
Rev.BB.AtEXP2for amplification/extraction of an arabidopsis germination-specific promoter5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3'
Fwd.BB.AtARP2for amplification/extraction of the arabidopsis ARP2 promoter5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3'
Rev.BB.AtARP2for amplification/extraction of the arabidopsis ARP2 promoter5'-AAG GCT GCA GCG GCC GCT ACT AGT CTT CTC CGA TTT CTA TAG AG-3'
barnase2.BB.fwdaddition of biobrick sites/ends to barnase gene5'-CCT TTC TAG AAT GGT ACC GGT TAT CAA CAC G-3'
BB.Barnase.Revaddition of biobrick sites/ends to barnase gene5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA TCT GAT TTT TGT AAA GGT CTG-3'
NLS.BB.Fwdaddition of biobrick sites/ends to the arabidopsis NLS5'-/5Phos/CTA GAT CTT CTG TTG TTC ATC CAT AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'
NLS.BB.Revaddition of biobrick sites/ends to the arabidopsis NLS5'/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA-3'
LacIn.BB.FwdAddition of NLS to the Lac Inhibitor gene to produce LacIN5'-CCT TGA ATT CGC GGC CGC ATC TAG AAT GAA AC AGT AAC GTT ATA CGA TGT C-3'
LacIN.BB.RevAddition of NLS to the Lac Inhibitor gene to produce LacIN5' AAG GTC GCA GCA GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA CTG CCC GCT TTC CAG TCG GGA AA-3'
BB.Gal4DBD.FwdAdding biobrick sites to Gal4 DNA Binding Domain5'-CTT TTC TAG AAT GAA GCT ACT GTC TTC TAT C-3'
BB.Gal4DBD.RevAdding biobrick sites to Gal4 DNA Binding Domain5'-AAG GCT GCA GCG GCC GCT ACT AGT CGA TAC AGT CAA CTG TCT TTG-3'
BB.Barstar.Fwdaddition of biobrick sites/ends to barstar gene5'CCT TTC TAG AAT GAA AAA AGC AGT CAT TAA C-3'
BB.Barstar.Revaddition of biobrick sites/ends to barstar gene5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA AGA AAG TATA GAT GGT GAT GTC-3'
2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP331 of 2 for sequencing V7 and V8 biobrick cloning site
VP342 of 2 for sequencing V7 and V8 biobrick cloning site