Team:Yale/LabNotebook/Week1

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Monday 6/7--defended project (yay!)

Wednesday 6/9--started in lab--got set up & started cultures of DH5alpha and LE392

Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran first copper growth assay in plate reader in PM w/three different ODs of each strain(4 hr. protocol)

Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--b/c allowed to overgrow?

Monday 6/14--Primer design, redo of Friday's growth assay

Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived

Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions

Thursday 6/17--more minimal media work and meeting, started BL21 culture

Friday 6/18--plasmid pSB74 should arrive! Transform all cell lines with it and with promoter and terminator (taken from kit). Make up plates with antibiotics. Help would be welcome :)