Team:Calgary/17 June 2010

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Thursday June 17, 2010

Dev, Chris, Jeremy: Today, Jeremy restreaked the construct of B0015-R0040, did a PCR on it. He then ran a gel electrophoresis on the five colonies that grew. Colonies 1 and 5 are presumed to have the part desired as observed from the gel picture shown on the right. He also wrote a thank you letter to BioAlberta from which we received $1000 today. Dev helped Emily in her construction of I0500-B0034 through the Quick Ligase procedure. We are using presumed I0500 because we are unsure of the quality of the growth. I0500 has so far, failed to grow consistently. Chris redid the construction of J13002-E0032 to test the YFP from the Registry as well as attempting a plasmid switch of J23032 to a kanamycin resistant plasmid.