User contributions

From 2010.igem.org

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• 19:48, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Beads/Procedure ‎ (→Procedure) (top)
• 11:10, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/RFPbead tests ‎ (→RFP Beads)
• 11:07, 18 October 2010 (diff | hist) File:GFP RFP in beads.png ‎ (uploaded a new version of "Image:GFP RFP in beads.png") (top)
• 11:06, 18 October 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/RFPbead tests ‎ (→Spectro-photometer data)
• 11:05, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/RFPbead tests ‎ (→Spectro-photometer data)
• 11:05, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/RFPbead tests ‎ (New page: =RFP Beads= Having established our ratio method of signal detection worked well in soil, we next wanted to combine this with our new bead technology to form and test a yet more advanced p...)
• 11:03, 18 October 2010 (diff | hist) N File:GFP RFP in beads.png ‎
• 10:54, 18 October 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/Experiments ‎ (→Experiments)
• 10:50, 18 October 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/signal soil ‎ (→Signal Visibility in Soil)
• 10:50, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/signal soil ‎ (New page: =Signal Visibility in Soil= For this project to work it was essential that a signal could actually be detected when in soil. To test this we mixed 'E. coli' constitutively expressing GFP ...)
• 10:40, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Experiments ‎ (→Experiments)
• 10:39, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/characterising ratio ‎ (New page: =Characterising the Ratio Method= ==0 - 10mM Assay== Having made our double transformants the next step was to characterise their response to Nitrate. We began by examining this between ...)
• 10:39, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Experiments ‎ (→Experiments)
• 10:38, 18 October 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/0-10mM RFPtest ‎ (→0 - 10mM Assay) (top)
• 10:37, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/0-10mM RFPtest ‎ (→0 - 2mM Assay)
• 10:32, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/0-10mM RFPtest ‎ (→Characterising the Ratio Method)
• 10:25, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/0-10mM RFPtest ‎ (→Characterising the Ratio Method)
• 10:17, 18 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/0-10mM RFPtest ‎
• 08:31, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/0-10mM RFPtest ‎ (New page: Having made our double transformants the next step was to characterise their response to Nitrate. We began by examining this between 0 and 10mM of Potassium Nitrate (in 2mM steps). 5mL sol...)
• 08:14, 18 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/adding RFP ‎ (New page: ==Implementing the Ratio Method== To solve the problem of not knowing whether a strong signal was due to many weakly fluorescing bacteria or a few strong ones we decided to implement a ra...) (top)
• 20:13, 17 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/difference solution ‎ (New page: ==Bead Difference in Solution== Having made our first beads containing the new biobrick BBa_K381001 we wanted to test whether there was a significant difference in fluorescence between th...)
• 20:12, 17 October 2010 (diff | hist) N File:Bead difference.jpg ‎ (top)
• 20:01, 17 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/making beads ‎ (→Making Beads)
• 19:58, 17 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/making beads ‎ (New page: ==Making Beads== To improve signal detection we decided to encapsulate our bacteria inside beads made of gellan gum and set about developing our own procedure. Research yielded several p...)
• 09:46, 13 October 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/0-10mM Assay ‎ (→Characterising the Biobrick - 0 - 10mM assay)
• 09:45, 13 October 2010 (diff | hist) N File:0 to 10 gfp average.png ‎ (top)
• 09:36, 13 October 2010 (diff | hist) N File:0 to 10 assay.png ‎ (assay 0 - 10) (top)
• 09:32, 13 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/0-10mM Assay ‎ (New page: ==Characterising the Biobrick - 0 - 10mM assay== Having established the biobrick was definitely switched on by 20mM of KNO3, we wanted to conduct a broader test of its behaviour across a ...)
• 09:27, 13 October 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/NO3test ‎
• 09:25, 13 October 2010 (diff | hist) N Team:BCCS-Bristol/Wetlab/NO3test ‎ (New page: Initial nitrogen vs not tests Having made our first biobrick, the next step was a series of basic experiments comparing its behaviour in an environment with nitrogen versus one without. ...)
• 17:01, 22 September 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/K381001 Construction ‎
• 16:59, 22 September 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Experiments ‎ (→Experiments)
• 11:36, 17 September 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/K381001 Construction ‎
• 11:35, 17 September 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/K381001 Construction ‎
• 11:33, 17 September 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/K381001 Construction ‎
• 11:30, 17 September 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/K381001 Construction ‎
• 11:22, 17 September 2010 (diff | hist) m Team:BCCS-Bristol/Wetlab/K381001 Construction ‎
• 11:16, 17 September 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/K381001 Construction ‎
• 11:00, 17 September 2010 (diff | hist) Nm Team:BCCS-Bristol/Wetlab/K381001 Construction ‎ (New page: Having decided on our biobrick design, our first task was to aquire the necessary parts and construct our first biobrick. BBa_I13522 was taken from well 8A kitplate 2 of the 2010 distribu...)
• 10:51, 17 September 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Experiments ‎ (→Experiments)
• 19:06, 16 August 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Notebook ‎ (→Notebook) (top)
• 13:14, 16 August 2010 (diff | hist) Team:BCCS-Bristol/Wetlab ‎
• 13:13, 16 August 2010 (diff | hist) Team:BCCS-Bristol/Wetlab ‎ (→Wet lab)
• 13:13, 16 August 2010 (diff | hist) Team:BCCS-Bristol/Wetlab ‎
• 13:11, 16 August 2010 (diff | hist) Team:BCCS-Bristol/Wetlab ‎ (→Wet lab)
• 13:04, 16 August 2010 (diff | hist) N File:Picture 018.jpg ‎ (top)
• 13:01, 16 August 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Notebook ‎ (→Notebook)
• 12:50, 16 August 2010 (diff | hist) Team:BCCS-Bristol/Wetlab/Notebook ‎ (→Notebook)
• 11:14, 11 August 2010 (diff | hist) N File:Labpic.jpg ‎ (top)
• 11:12, 11 August 2010 (diff | hist) Team:BCCS-Bristol/Wetlab ‎ (→Wet lab)

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