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From 2010.igem.org

Assembly of biobricks in pSB1C3-backbone for submission to parts registry by Maarten

O/N cultures of transformants were mini prepped with Sigma Aldrich mini prep kit and control restrictions were performed.

Control restrictions with EcoRI and PstI on constructs:

2 µL construct

2 µL Fast Digest Buffer

0,5 µL EcoRI Fast Digest

0,5 µL PstI Fast Digest

15 µL MQ

Construct	Expected band sizes (bp)
pSB1C3-C	2031, 848
pSB1C3-DC	2031, 872
pSB1C3-E	2031, 317
pSB1C3-H	2031, 302
pSB1C3-Sortase	2031, 689
pSB1C3-RFP	2031, 1069

The gels were not very conclusive but samples were sent for sequencing on 15 September.

Peter
Chaplin detection
Samples from the dry freezer were prepared using TFA.
The samples were diluted with 1 mL Q-H20 and put on gels:
Gel 1: chppt=e deltapt=e bpte e1pte enpte chste deltaste bste e1ste
Gel 2: enste deltapt=4 bpt=4 e1pt=4 enpt=4 deltast=4 bst=4 ent=4
Gel 3: est=4 deltadt=e bdt=e e1dt=e endt=e 1 2 3
Gel 4: 4 e1dt=e bdt=e e1nt=e e1pt=e bpt=e e1st=e b1st=e