Team:Stockholm/1 October 2010
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Contents |
Andreas
LB agar plates
- 20 x 100 μg/ml Amp LB agar plates
Assembly of His⋅SOD⋅cCPP constructs
Digested pMA.His⋅SOD constructs for later assembly into cCPP plasmids, digested by Johan.
Digestion
pMA.His⋅ SOD | |
---|---|
10X FastDigest buffer | 3 |
DNA (3 μg) | 7.5 |
dH2O | 17.5 |
FD EcoRI | 1 |
FD AgeI | 1 |
30 μl |
- Incubation: 37 °C, 0:30
- Inactivation: 80 °C, 20 min
Stored in -20 °C for later ligation.