Team:Stockholm/1 October 2010

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Contents

Andreas

LB agar plates

  • 20 x 100 μg/ml Amp LB agar plates

Assembly of His⋅SOD⋅cCPP constructs

Digested pMA.His⋅SOD constructs for later assembly into cCPP plasmids, digested by Johan.

Digestion

  pMA.His⋅ SOD
10X FastDigest buffer 3
DNA (3 μg) 7.5
dH2O 17.5
FD EcoRI 1
FD AgeI 1
  30 μl
  • Incubation: 37 °C, 0:30
  • Inactivation: 80 °C, 20 min

Stored in -20 °C for later ligation.