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Team:HokkaidoU Japan/Notebook/September30
From 2010.igem.org
since 13 Jul 2010
since 1 Aug 2010
- Digestion and Ligation of Arabinose Promoter,T3SSsignal and pSB1T3
- Transformation
parts information
Arabinose Promoter 1210bp(+primer:1259bp)
3-20B pSB2K3
RBS+T3SSsignal 583bp(+primer:636bp)
pSB1T3 2463bp(+primer:2504bp)
Digestion and Ligation of Arabinose Promoter,T3SSsignal and pSB1T3
| Reagent | Amount |
|---|---|
| 10x H buffer | 2 uL |
| DW | 1.6 |
| pSB1T3 | 1 |
| BSA | 2 |
| EcoR I | 0.2 |
| Pst I | 0.2 |
| Total | 20 uL |
| Reagent | Amount |
|---|---|
| 10x H buffer | 2 uL |
| DW | 11.7 |
| Promoter | 3.5 |
| BSA | 2 |
| EcoR I | 0.5 |
| Spe I | 0.3 |
| Total | 20 uL |
| Reagent | Amount |
|---|---|
| 10x M buffer | 5 uL |
| DW | 32.8 |
| T3SSsignal | 2.5 |
| BSA | 5 |
| Xba I | 4.5 |
| Pst I | 0.2 |
| Total | 50 uL |
