Talk:Team:IvyTech-South Bend/27 August 2010

From 2010.igem.org

8/27/10

We will be running a gel to determine if our DNA sample was successfully electroplated. 1) In microwave I melted TAE buffer/gel on the beverage setting and let melt the combined both bottles of TAE buffer/gel. 2) Set up the gel box and electric source and taped up the gel box. 3) We will be using TAE buffer made by Joe Hull/Me on 4/9/10 4) Prof. T added 5 mL Ethidum Bromide into 40 mL of TAE buffer/gel then poured into gel box let sit and harden for DNA run.

== 8/27/10

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Today we will be electroplating part KBBa_3131010 8/27/10 J.H. into electrocomp. Cells for further experiments. 1) Cleaned lab station with disinfectant spray and wiped clean. 2) Set up an ice water bath placed sapl., 900 mL of Sob media in a 1 mL sterile tube, and other saple. from yesterday on ice. 3) Plugged in and turned on electroporator and set to bacteria screen read ECl 4) Removed a centrifuge tube containing electrocomp. cells from the -80 C freezer and thawing at pocket temp. made by prof. Twaddle. 7/23/10 Now thawed and placed on ice bath. 5) Curette is also placed in ice bath to cool. 6) Protocol has been changed we will electo. cells wtihout DNA, cells with DNA and streak cells without electro. Results we believe that the Amp could have been destroyed by the Agar due to too high of temp when added. We are going to make and streak new plates. --Rchamberlin 18:29, 30 September 2010 (UTC)