Team:Cambridge/Gibson/MasterMix

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	Volume/µl
Taq ligase (40u/µl)	50
5x isothermal buffer	100
T5 exonuclease (1u/µl)	2
Phusion polymerase (2u/µl)	6.25
Nuclease-free water	216.75
	=375

Master Mix is 1.33x concentrated

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