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From 2010.igem.org

Contents 1 1：DNA extraction from gels 2 2：DNA Ligation 3 3:PCR 4 4：DNA extraction 5 5:Electrophoresis 6 6：DNA extraction 7 7：DNA Digestion

1：DNA extraction from gels

＜Member＞
mizuki

＜Sample＞
Digestion products

• 2
• 4
• 5
• 8

(After electrophoresis)

＜Protocol＞
See Protocol 4

2：DNA Ligation

＜Member＞
mizuki

＜Sample＞

• 4
• 5

(after digestion)

＜Protocol＞
See Protocol 3

3:PCR

<Member>
mizuki

<Sample>(temperature in annealing)

• 5
• 7
• 9
• 10

<Protocol>
See Protocol 1

4：DNA extraction

＜Member＞
mizuki

＜Sample＞

• 7-8 (9/17)

＜Protocol＞
See Protocol 4

5:Electrophoresis

<Member>
mizuki

<Sample>
PCR products (9/25)

• 5
• 7
• 9
• 10

Ligation products (9/25)

• 4-5

<Protocol>
See Protocol 8

<Result>

6：DNA extraction

＜Member＞
mizuki

＜Sample＞
PCR products

＜Protocol＞
See Protocol 4

7：DNA Digestion

＜Member＞
mizuki

＜Sample, Materials＞

• 7-8 (9/6)
• 10 (9/25)

＜Protocol＞
See Prptpcpl 9