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From 2010.igem.org

Contents 1 Overall project 2 Project Details 2.1 Part 2 2.2 The Experiments 2.3 Part 3 3 Results

Overall project

Standardization of the Rhamnosiltransferase 1 gene complex (rhlAB) into a Biobrick-friendly for rhamnolipid production in E. coli.

L. Coronado1, E.B. Gómez3, 4, C. De La Guardia1, C. Restrepo1, Y. González1, N. Gómez 3, N. Tayler1, L. Pineda1, Y. Suarez1, G. Ureña2, L. Abrego1, E. Aguilar2, C. Monteza3, S. Pinzón4, O. Sanjur4, C. Spadafora1 y P. Nee5#

1Universidad de Panamá, 2Instituto de Investigaciones Científicas y Servicios de alta Tecnología, 3Universidad Tecnológica de Panamá, 4Smithsonian Tropical Research Institute, 5, #MIT alumnus

1. Corresponding autor

• Experimentos realizados en INDICASAT AIP y STRI, República de Panamá

@Financiado por SENACYT, INDICASAT y STRI

There is considerable interest among bio-industries in bioremediation products such as Rhamnolipids. Rhamnolipids as biosurfactants are important in the remediation of oil spill areas. The cleanup of the Exxon Valdez oil spill using rhamnolipids as biosurfactants was too expensive and complicated, therefore impractical for large-scale bioremediation. However, with advances genetic engineering and synthetic biology offer a viable solution to oil spill pollution clean up. In this work we use genetic engineering as a tool to integrate genetic parts by the biobrick assembly standard protocol of iGEM to develop a biobrick-friendly for rhamnosiltransferase 1 complex (rhlAB) gene expression in Escherichia coli for standardized rhamnolipid production. Our biobrick integrates a promoter, a RBS (ribosomal binding site), our gene sequence of rh1AB isolated from Pseudomonas aeruginosa, a GFP reporter and a terminator. All the parts fit into a plasmid backbone that can be transformed into E. coli strains, which can then produce rhamnolipids.

Project Details

Part 2

The Experiments

Part 3

Results