Team:Alberta/Building Parts

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Contents

Building Parts

May
MTWTFSS
          [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_May_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_May_2010&action=edit 2]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_May_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_May_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_May_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_May_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_May_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_May_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_May_2010&action=edit 9]
[http://2010.igem.org/Team:Alberta/10_May_2010 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_May_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_May_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_May_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_May_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_May_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_May_2010&action=edit 16]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_May_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_May_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_May_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_May_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_May_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_May_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_May_2010&action=edit 23]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_May_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_May_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_May_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_May_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_May_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_May_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_May_2010&action=edit 30]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/31_May_2010&action=edit 31]
June
MTWTFSS
  [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_June_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_June_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_June_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_June_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_June_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_June_2010&action=edit 6]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_June_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_June_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_June_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/10_June_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_June_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_June_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_June_2010&action=edit 13]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_June_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_June_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_June_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_June_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_June_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_June_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_June_2010&action=edit 20]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_June_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_June_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_June_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_June_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_June_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_June_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_June_2010&action=edit 27]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_June_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_June_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_June_2010&action=edit 30]
July
MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_July_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_July_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_July_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_July_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_July_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_July_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/10_July_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_July_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_July_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_July_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_July_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_July_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_July_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_July_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_July_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_July_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_July_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_July_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_July_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_July_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_July_2010&action=edit 25]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_July_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_July_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_July_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_July_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_July_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/31_July_2010&action=edit 31]
August
MTWTFSS
            [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_August_2010&action=edit 1]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_August_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_August_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_August_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_August_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_August_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_August_2010&action=edit 8]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_August_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/10_August_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_August_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_August_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_August_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_August_2010&action=edit 15]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_August_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_August_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_August_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_August_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_August_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_August_2010&action=edit 22]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_August_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_August_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_August_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_August_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_August_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_August_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_August_2010&action=edit 29]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_August_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/31_August_2010&action=edit 31]
September
MTWTFSS
    [http://2010.igem.org/wiki/index.php?title=Team:Alberta/1_September_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/2_September_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/3_September_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/4_September_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/5_September_2010&action=edit 5]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/6_September_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/7_September_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/8_September_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/9_September_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/10_September_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/12_September_2010&action=edit 12]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/13_September_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/14_September_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/15_September_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/16_September_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/17_September_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/18_September_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/19_September_2010&action=edit 19]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/20_September_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/21_September_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/22_September_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/23_September_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/24_September_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/25_September_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/26_September_2010&action=edit 26]
[http://2010.igem.org/wiki/index.php?title=Team:Alberta/27_September_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/28_September_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/29_September_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=Team:Alberta/30_September_2010&action=edit 30]

10-05-2010

PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the A/B' ends or the B/A' ends.

Recipe:

1uL p1003 (approx. 1ng)
2.5uL prA_p1003+
2.5uL prB'_p1003-
5uL 10X PCR buffer
1uL 10uM dNTPs
2uL 50uM MgCl~2~
0.5uL Taq polymerase
35.5uL MilliQ H~2~O

Program:



11-05-2010

June 3, 2009

  1. Plasmid transformed = pSB1AC3 (TEST)
    • Note: team members were of mixed opinion as to whether the transform was likely due to low availability of DNA - DNA was split between a number of transformation reactions for at least 2 different DB3.1 strains. Plates incubated at 30 degrees Celsius overnight, temp. raised to 37 degrees approx. 10:30 a.m.

June 5, 2009

  1. Tet plates made
    Recipe for 200 ml (approx. 10 plates):
    2.2 g agar in 200 ml fresh LB
    Note: do not re-autoclave LB, it will caramelize!
    Recipe for 200 ml LB:
    a) 1 g yeast extract
    b) 2 g peptotryptone
    c) 2 g NaCl
    d) 200 ml water
  2. Autoclaved at 11:20 a.m., picked up at 1:15 p.m. and cooled in water bath at 37 degrees Celsius
  3. Added 200 microlitres tetracycline stock (from -20 freezer) to final concentration of 20 microgram/ml
  4. Swirl and poured into prepared, labeled plates
    • Note: label on plates reads June 3, 2009 but should be June 5, 2009 (8 plates in total - either I overpoured or these plates are a bit larger than I was used to)
  5. Inverted and put in 37 degree incubator to dry

June 8, 2009

  1. Bacteria grown to log phase using sterile inoculating loop to inoculate approx. 200 ml of LB media with bacteria from 2 plates (approx. 3 colonies)
  2. Bacterial liquid culture placed in shaker at 10:51 a.m.

June 9, 2009

  1. Digested miniprepped gel with EcoRI and SpeI
  2. Running gel to see if correct plasmid was purified - should expect to see bands at ~3200 bp
  3. DNA Ladder made - 6 microlitres of stock used
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