Lab work

BioBrick stocks

Today we performed a Qiagen Mini-prep plasmid isolation of the vectors we grew overnight. We saved 3 mL of the bacterial cells to make -80 °C stocks.

The following plasmid concentrations were obtained:

BioBrick	Concentration (ng/μL)
pSB3T5	266.0
pSB1T3	414.0
pSB1C3	284.7
pSB3C5	329.3
pSB1K3	270.1
pSB1A3	271.9
I13401	232.1

Ramon & Hugo in the lab

Thias doing labwork

Characterization of Anderson RBS sequences

Next to that we also started digesting the first parts we will be characterizing.

Spring Workshop

Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep.