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AUGUST: WEEK 4
August, 23rd
Colony PCR as screening for ligations I31..I41. For each plate three colonies were picked and PCR amplification was performed. At the same time colonies were let grow in 750 ml LB+Amp in order to be ready to make glycerol stocks for those with right amplicons.
 Colony PCR and gel run as screening for ligations I31..I41 Only I31, I33, I34, I35, I37, I38, I41 are positive without any doubt (we took I31-1, I33-1. I34-1, I35-1, I37-1, I38-3, I41-1), while I39 were all wrong and we weren't sure about I32, I36 and I40 (we made however glycerol stocks for I32-2, I36-2, I40-1).
We decided to repeat colony PCR for I32, I36, I39 and I40 next day.
August, 24th
August, 25th
August, 26th
August, 27th
August, 28st
August, 29nd
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