"
Team:Stockholm/19 August 2010
From 2010.igem.org
Revision as of 10:10, 19 August 2010 by AndreasConstantinou (Talk | contribs)
| Home | Project Idea | Lab Work | Results | Modelling | Team | Follow us on  and 
| 2010.igem.org 
|
Andreas
Site-directed mutagenesis of SOD & yCCS
Sequencing results
Received the sequencing results for m-SOD 1, m-SOD 2, m-yCCS 1 and m-yCCS 4 from Eurofins. Ran a nucleotide BLAST (BLASTn) alignment to verify successful site-directed mutagenesis.
- SOD 1: Failed (BLASTn results)
- Mutation still present
- SOD 2: Success (BLASTn results)
- Mutation removed
- yCCS 1: Success (BLASTn results)
- EcoRI site removed
- yCCS 4: Success (BLASTn results)
- EcoRI site removed
SOD 2 sample and glycerol stock will be saved, while SOD 1 will be discarded.
Unfortunately, BLAST results also discovered 4 (!) identical mutations in both yCCS sequences. A protein BLAST (BLASTp) alignment of the translated yCCS sequences revealed all four mutations as silent mutations.
