Team:LMU-Munich/Cut'N'survive/Schedule/PCR key

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Cut'N'Survive System Functional Principle Sequences Schedule Jump-or-Die System Functional Principle Sequences Schedule ProSearch System Schedule Functional Principle Sequences Schedule picture gallery

1TreF 56°C: #Number: consecutive numbering on PCR plan #Tre: Abbreviation of gene of interest #F: Forward Primer #56°C: Annealing temperature (4+2 rule) 4TevMutEPR : #Mut: Mutagenesis Primer #E: EcoRI digestion site #P: PstI digestion site #R: Reverse Primer Also: #S: SpeI digestion site

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