"
Team:ETHZ Basel/Biology/Status
From 2010.igem.org
generation of subparts
| Protein | primers | PCR product | library of transformants | single colonies | storage vector | insert | |
|---|---|---|---|---|---|---|---|
| che proteins | CheY-A | recieved | purified on stock | on agar plate | not available | purified on stock | on stock |
| CheY-C | recieved | purified in stock | on agar plate | not available | purified on stock | on stock | |
| CheB-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock | |
| CheB-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock | |
| CheR-A | recieved | purified in stock | on agar plate | 3 on agar plate | digest has to be redone | ||
| CheR-C | recieved | purified in stock | on agar plate | 3 on agar plate | digest has to be redone | ||
| localizers | TrigF-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | |
| MreB-C | recieved | purified in stock | in process | ||||
| tetR-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock | |
| couplers | PIF3-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | |
| PIF3-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | ||
| PhyB-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | ||
| reporters | cyFP-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock |
| cyFP-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock |
generation of biobricks
testing of che-fusions
We're currently trying to establish an assay to test for the chemotactic behavour of E. coli. A first try with a "syringe test" failed. We'll redo the test and are looking for other possibilites. Maybe a swarm test?
testing of localicer-fusions
We are planning to use a fluorescence microscope in order to visualize the distribution of localizer-cyFP fusions within a single E. coli.
testing of couplers
We're planning to use the setup as for the testing of the localizers. In this case we're going to use the following biobricks:
- localizer-PhyB and PIF3-cyFP or
- localizer-PIF3 and PhyB-cyFP
Images should look as in testing of localizers
Preliminary clarificaiton of the suitability of the approach are to be done.
testing of biobricks
bringing E. lemming alive
timetable
