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AUGUST
| DATE | ACTIVITY |
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| August, 1st |
44° Bio-Lab - I20 and I21 plates showed colonies. Some were picked, inoculated and grown in proper medium.
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| AUGUST: WEEK 1 |
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| August, 2nd |
45° Bio-Lab - I20-1/2/3 and I21-1/2/3 ligations sent sequencing.
<partinfo>BBa_R0062</partinfo> and <partinfo>BBa_K081009</partinfo> resuspended from iGEM 2010 Distribution Kit.
Trasformation of pAH123 and <partinfo>BBa_J72008</partinfo> helper into MC1061 and MG1655.
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| August, 3rd |
46° Bio-Lab -
<partinfo>BBa_R0062</partinfo> and <partinfo>BBa_K081009</partinfo> single colony pick from plates.
MC1061 and MG1655 colonies transformed with helpers were picked from plates and inoculated and and incubated in proper medium.
PCR from colony (MG1655 and MC1061) using synthesized primers to check them and to verify a negative control for integration.
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| August, 4th |
47° Bio-Lab - Glycerol stocks for MC1061 and MG1655 strains transformed with pAH123 or <partinfo>BBa_J72008</partinfo> helper plasmids. Miniprep of MC1061 and MG1655 to check again the presence of helper plasmids. Digestion with SpeI and gel run. We got positive samples but a bad run. So we picked again from plates, this time single colonies.
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| August, 5th |
48° Bio-Lab -
Miniprep of newly picked colonies MC1061 and MG1655, digestion with SpeI and gel run.
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| August, 6th |
49° Bio-Lab -
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| August, 7th |
50° Bio-Lab -
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| August, 8th |
51° Bio-Lab -
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| AUGUST: WEEK 2 |
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| August, 9th |
52° Bio-Lab -
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| August, 10th |
53° Bio-Lab -
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| August, 11th |
54° Bio-Lab -
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| August, 12th |
55° Bio-Lab -
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| August, 13th |
56° Bio-Lab -
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| August, 14th |
57° Bio-Lab -
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| August, 15th |
58° Bio-Lab -
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