"

From 2010.igem.org

Contents 1 June 21 2 June 22 3 June 23 4 June 24 5 June 25

June 21

Sent to the sequencing center:

              Base Pairs
 1)C3RFP      681
 2)K091146    1266
 3)K112022    1499
 4)K145150    66
 5)R0063      151
 6)R1062      56
 7)K081007    796

• We made 1 liter of LB media.

June 22

• Flasks Minipreped:

  -J23108
  -J23116
  -J37033
  -K091107
  -C0261
  -K274100
  -C3RFP
  -K112808

• All the minipreps were cut with the following enzymes:

  -J23108 (SP)
  -J23116 (SP)
  -J37033 (XP + phosphatase)
  -K091107(SP)
  -C0261  (XP + phosphatase)
  -K274100(XP + phosphatase)
  -C3RFP  (EX)
  -K112808(ES + phosphatase)

• Ligations:

- J23108 + J37033
- J23116 + J37033
- K091107 + C0261
- K091107 + K274100
- C3RFP + K112808

• Transformations:

- B0010    Plate 1 Well 13D
- R0010    Plate 1 Well 1D
- K081007  Plate 2 Well 10J
- J24679   Plate 1 Well 7L

• We also did a PCR on K081007 + R0010, J23116 + J37033, and J23108 + J37033.

June 23

• Transform today:

  - R0061  Plate 1 Well 14P
  - R0062  Plate 1 Well 6O
  - R0063  Plate 1 Well 8A
  - K091146 Plate 2 Well 8H

• Digests:

 - K091107 (SP)
 - R0010   (SP)
 - J24679  (ES)
 - J23108  (SP)
 - J23116  (SP)
 - CO261   (XP)
 - K081107 (XP)
 - B0010   (EX)
 - J37033  (XP)
 - K274100 (XP)

• Sent to get sequenced:

 1) R0010
 2) B0010
 3) K081107
 4) J24679

June 24

Gel Order:

 1) Ladder
 2) J24679
 3) K091107
 4) J37033
 5) J23108
 6) K274100
 7) B0010
 8) R0010
 9) K081107
 10)CO261
 11)J23116

RE-DIGEST:

- CO261
- K081107

RE-Transform:

- J37033
- K091107

• Sent to sequencing:

1) R0061
2) R0062
3) R0063
4) K091146

June 25

• Sent to sequencing:

1) K091107
2) J37033

• Digests:

 - K091107 (SP)
 - J23108  (SP)
 - J23116  (SP)
 - CO261   (XP)
 - R0010   (SP)
 - K274100 (XP)
 - J37033  (XP)
 - J24679  (ES)
 - K081007 (XP)

• Ligations:

 - K091107 + K274100
 - J23108  + J37033
 - J23116  + J37033
 - C0261   + J24679 
 - R0010   + K081007

The 4 that were sent to get sequenced yesterday were ran on a gel. The gel order is provided below:

- Ladder
- K091146
- R0061
- R0062
- R0063