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Team:Cambridge/Notebook/Week4
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Revision as of 09:30, 4 August 2010 by EmilyKnott (Talk | contribs)
Notebook: Week 4
Monday
- Wet work
- Transformed competent cells using plasmid phK724 from Jim Slock (thanks!)
- Grew up E. coli containing phK555 in 5ml LB broth in rotating 37 C incubator overnight
- Dry work
- Finalising sequencing requirements
- Dismissed PPK
- Decided to include Luciola cruciata
- Further Wiki work
- Finalising sequencing requirements
Tuesday
- Wet Work
- Made competent cells of 4 different hns mutants: W3110 hns93-1, BW25113 Δhns::kan, W3110 hns-205::Tn10, GM230 hns-205::Tn10
- Now stored in the -80 C freezer for later use
- Dry Work
- Organising sequences for synthesis
Wednesdy
Thursday
Friday
Saturday
Sunday
