Team:ETHZ Basel/Biology

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As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).

Currently we are working on putting all the BioBricks into a storage vector. The image () shows all the constructs we plan to clone.