"

From 2010.igem.org

Alkane Degradation

With a number of yesterday's colonies a digestion was done to check if they contain the desired insert. The table below describes the digestion procedure, as well as the gel lane description. (Digestion was done in 10μl end-volume)

#	Contents	Enzyme 1	Enzyme 2	Buffer	BSA	Expected size(s) (bp)
1	Smartladder (5μl)					n/a
2	007T (2)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	1341, 2455
3	008T (4)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	276, 2455
4	009T (6)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	285, 2455
5	009T (7)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	285, 2455
6	010T (9)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	1382, 2455
7	017T (14)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	855, 2455
8	Empty
9	007T (2)	1 μl PstI	1 μl PvuII	H (Boehringer)	no	2529,983,251
10	008T (4)	1 μl AatII	0.5 μl KpnI	A (Boehringer)	1 μl	271, 2427
11	009T (6)	0.5 μl KpnI	1 μl NruI	B (Boehringer)	1 μl	598, 2109
12	009T (7)	0.5 μl KpnI	1 μl NruI	B (Boehringer)	1 μl	598, 2109
13	010T (9)	1 μl EarI	0.5 μl PstI	1 (Biolabs)	no	1075, 2729
14	017T (14)	0.5 μl AdhI	0.5 μl SpeI	4 (Biolabs)	no	428, 2849
15	EZ load (ladder)					n/a

It seems (from the gel) that BioBrick 007T is the correct one, and the others don't contain the desired insert.