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From 2010.igem.org

Contents 1 Colony PCR of Genomic DNA 1.1 Aim: 1.2 Materials: 1.3 Protocol: 1.3.1 Conditions in ThermoCycler:

Colony PCR of Genomic DNA

Aim:

To determine whether the genes have been inserted into the plasmid of B. Subtilis 168.

Materials:

• Pipette
• Microfuge
• Microtubes
• Distilled H2O
• Nucleotide DNTP
• 5x GoTaq buffer
• Template DNA (B. Subtilis ATCC 6633, 1:1 and 1:2)
• Forward and reverse primers

Protocol:

1. For the full protocol, please refer to Colony PCR in Protocol List.

Conditions in ThermoCycler:

1. Melting temperature, Tm used for Anneal step is 59°C.