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From 2010.igem.org

Eppy

Pieter just got a little present from Mariska van Ham :-) He's so happy with is eppy!

Today Nadine & Luke are learning how to work with HTML.

Lab work

BioBrick stocks

A number of Biobricks from the DNA distribution plates were suspended yesterday. Hugo and Kira did a transformation with 2 μL of the following BioBricks (antibiotic):

• pSB3T5 (Tetracycline)
• pSB1T3 (Tetracycline)
• pSB1C3 (Chloramphenicol)
• pSB3C5 (Chloramphenicol)
• pSB1K3 (Kanamycin)
• pSB1A3 (Ampicillin)
• I13401 (Ampicillin)

Because our own competent cells were not good enough we used commercially available competent Top10 cells (Invitrogen). The cells were plated on LB-agar with the appropriate antibiotic. Biobricks plated We will have to wait until tomorrow to see if they were successful or not. Hopefully we will see some colonies tomorrow. Wish us luck!

Thias PCRed some BioBricks with various primer concentrations and at different temperatures to see which PCR method would work best. The PCR products were tested on a gel, and we saw a difference between the primer concentrations and the temperatures used.