deleting allergens from fruits and vegetables
Allergies to fruits and vegetables are (increasingly) common, affecting millions of people around the
world with symptoms ranging from mild itchiness to life-threatening anaphylaxis. Allergy is caused by an
inappropriate immune response to harmless proteins present in the environment. Several common food allergens
are structurally similar to pollens that cause seasonal allergies and are present in a wide range of fruits
and vegetables. Many of these proteins have been knocked down in plants using RNA interference, leading to
plants with reduced allergenicity. As part of our iGarden project we are designing modular BioBrick
intron-containing self-complementary hairpin forming RNA (ihpRNA) and artificial microRNA (amiRNA)
constructs for the targeted knockdown of proteins with homology to allergens in arabidopsis
and strawberry, as well as designing ihpRNA and miRNA constructs
against allergens in a range of other plants common in home gardens, including lettuce, carrots, celery, tomato,
and several herbs. Our goal is to use genetic engineering to make food safer, and to specially tailor gardens
to the needs of each person with a different set of allergies.
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