2010.igem.org/Team:Harvard/vectors/vectors

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meet the vectors

Our vectors are a modification of the pORE agrobacterium vector system developed by Coutu et al at Agriculture and Agrofood Canada. This vector system is designed for agrobacterium mediated plant transformation, and is a binary system with the vector containing the site for foreign constructs and the appropriate flanking regions to be recognized for introduction to the plant. The virulence genes are present on a separate vector contained in the agrobacterium, separate from the TDNA.

To modify the pORE series binary vectors to fit the biobrick standard, we digested the vectors with. We worked with two of each of the open, reporter, and expression series vectors, creating a total of six biobrick standard vectors suitable for agrobacterium mediated plant transformation.


open series   <a href="../images/open_large.png" id="single_image" style="font-size:12px">click to enlarge</a>

<a href="../images/open_large.png" id="single_image"> <img src="../images/open_small.png" width="300px" border=0> </a>


The open series vectors are designed for general insertion of a construct. We modified the vectors pORE O1 and pORE O2. Each has a different antibiotic resistance.
reporter series   <a href="../images/reporter_large.png" id="single_image" style="font-size:12px">click to enlarge</a>

<a href="../images/reporter_large.png" id="single_image"> <img src="../images/reporter_small.png" width="300px" border=0> </a>


The reporter series vectors contain a reporter on the trailing end of the multiple cloning site such that expression of the reporter follows that of the inserted construct. We modified the vectors pORE R1 and pORE R3. pORE R1 contains the gusA reporter, and pORE R3 the smgfp reporter.

expression series   <a href="../images/expression_large.png" id="single_image" style="font-size:12px">click to enlarge</a>

<a href="../images/expression_large.png" id="single_image"> <img src="../images/expression_small.png" width="300px" border=0> </a>


The expression series vectors contain a promoter preceding the multiple cloning site such that the inserted construct can be easily expressed through activation of the contained promoter. We modified the vectors pORE E3 and pORE E4. Both contain the ENTCUP2 promoter, though each has a different plant resistance gene (E3 contains ____ and E4 _____)







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