"

From 2010.igem.org

After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.

We also booked a hotel in Boston!

Lab work

Emulsifier Pieter worked on the emulsifying activity assay. Four samples were tested:

1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.

Sample	Amount of SDS (%)	Hexalkane (mL)
1	0	0
2	0	0.02
3	0.01	0.02
4	1	0.02

2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.

3. Shake 1 hour with 200 strokes/min at 30°C

4. Measure absorbance OD600

Sample	A600
1	0
2	0
3	0.4
4	0.2