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Team:Queens-Canada/23 June 2010
From 2010.igem.org
June 23, 2010
PCR
Steve and Yuli
pHsp-3
Program run using HIFI with an extension time of 45 s and annealing temperature of 58 °C.
2010 PCR Hao, Louis and Mary
pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP
Program run using HIFI with an extension time of 30 s and annealing temperature of 57 °C.
Gel Electrophoresis
Yuli and Nelson
pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP, pHsp-3::eGFP
Used 0.4 g agarose in 65 mL TBE. Gel run for 40 mins at 100 V.
Heat Shock
Hao and Thai
pIC576, pIC517, pIC518
Plates stored in 37 °C room overnight.
DNA Rehydration
Hao and Thai
BBa_I13522
Heat Shock
Thai, Mike and Mary
BBa_I13522
Plates stored in 37 °C room overnight.
PCR
Thai, Louis and Mike
pHsp-3
Program run using HIFI with an extension time of 90 s and annealing temperature of 58 °C.
