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HKU-Hong Kong/20 July 2010
From 2010.igem.org
Work schedule 20 July 2010
The concentration of BBa_k112808 obtained with the method previously worked on is too low.We turned to PCR for obtaining BBa_k112808.
The PCR products as well as the plasmid pET28a were then treated with NdeI/NcoI+XhoI.
