Team:HokkaidoU Japan/Notebook/September29

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Electrophoresis of Yesterday Samples

Electrophoresis of concentrated GFP-double terminator(T3SS signal wasn't seen)

electrophoresed 6 uL Marker λ/HindIII EcoRI and 1 uL of two yesterday samples.T3SS signal wasn't observed.

Colony PCR of Arabinose Promoter + RBS + GFP + double terminator

PCRed 5 colony.

PCR mix

Reagent	Amount
Quick Taq	25 uL
EX-F	0.5 uL
PS-R	0.5 uL
Total	26 uL

PCRed according to the table below.98C and 68C for 35 cycles.

temp	time
94C	2 min
94C	30 sec
68C	90 sec
4C	hold

erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI.

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