Team:DTU-Denmark/Parts

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Revision as of 11:51, 27 October 2010 by LisaBlanc (Talk | contribs)

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BioBricks submitted

Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR

  • BBa_K374008

This part contains the promoters: pRGifsy1 and pRMGifsy1. Downstream of the pRMGifsy1 promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRMGifsy1 and a repressor of the pRGifsy1.

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GogR repressor. BBa_K374008 can be combined with BBa_K374010 hence allowing expression of your inserted BioBrick.

This part has been characterized, for more information look here or in parts-registry, BBa_K374008

Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR

  • BBa_K374009

This part contains the promoters: pRGifsy2 and pRMGifsy2. Downstream of the pRMGifsy2 promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRMGifsy2 and a repressor of the pRGifsy2.

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick.

This part has been characterized, for more information look here or in parts-registry, BBa_K374009

Antirepressor AntO from Gifsy1 phage

  • BBa_K374010

AntO inactivates the repressor from Gifsy1 phage, resulting in transcription from the pRGifsy1 promoter. AntO works by direct non-covalent binding to gogR and prevents its DNA binding activity.

This regulatory biobrick can be used in a biological circuit. It can de-repress the effect of the repressor from BBa_K374008 allowing expression from the pRGifsy1 promoter.

For more information look in parts-registry, BBa_K374010

Lamda nutRsite - N protein utilization site

  • BBa_K374005
Lambda nutR site - N utilization site + Tr1 rho dependent terminator.

In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element. This part contains the lambda nutR site which together with the N-protein will suppress termination downstream regardless of what terminator is used.

This regulatory biobrick can be used in a biological circuit. It can supress any termination. If place upstream of the terminator, and applied together with BBa_K374006

For more information look in parts-registry, BBa_K374005


Parts

New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.

<groupparts>iGEM010 DTU-Denmark</groupparts>