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Team:Queens-Canada/29 June 2010
From 2010.igem.org
June 29, 2010
Liquid Culture
Thai and Hao
BBa_I13522(Amp)
Incubated at 37 °C starting at 11:00.
Gel Electrophoresis
Thai and Hao
pMec-7, pRab-7, pSip-1
PCR
Basia and Thai
pIC576-1a, pIC57-1b, pIC518-1a, pIC518-b.
Program run using HIFI with an extension time of 30 s and annealing temperature of 61 °C.
PCR
Yuli and Steve
pSip-1:::eGFP extract, pRab-7:::eGFP extract, BBa_I1408
Program run using HIFI with an extension time of 165 s and annealing temperature of 58 °C.
PCR
Thai NpHR, ChR2
Program run using HIFI with an extension time of 30 s and annealing temperature of 60 °C.
PCR
Hao unc-22 C, unc-22 A
Program run using HIFI with an extension time of 32 s and annealing temperature of 58 °C.
Gel Electrophoresis
Hao
pIC576, pIC518, NpHR, ChR2, unc-22 C, unc-22 A
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.
