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From 2010.igem.org

Arabidopsis grown on 0.5 MS media

For one liter of 0.5 MS:

2.165 gm MS salt + 15 gm sucrose (1.5%) + 12 gm agar (1.2%). Adjust the pH to 5.7 with I N NaOH.

Procedure

Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7. LASTLY, add agar.

AutoClave Notes:

Use container that is twice as large as solution volume (ex. for 500 mL solution one should use 1000mL flask). Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.