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Team:Queens-Canada/parts
From 2010.igem.org
Part Structure
Show/hide parts as entered in the [http://partsregistry.org Registry].
Note: The following list is tentative, and better reflects our goals rather than our current accomplishments. We are still in the process of isolating and characterising these components.
General
Promoters
| Name | Description | Strength |
|---|---|---|
| sip-1 | Heat shock | |
| rab-7 | Constitutive | high |
| mec-7 | Mechanosensory neurons | |
| hsp-3 | Heat shock | |
| gpd-2 | Constitutive | very high |
| unc-47 | GABA-using (gabergic) neurons |
Coding Sequences
| Name | Description |
|---|---|
| GFP | Enhanced Green Fluorescent Protein. From biobrick BBa_I714891. Proof of concept to see if genes can be imported from bacteria. |
| eGFP | Enhanced Green Fluorescent Protein. From Chin-Sang plasmid pIC691. |
| mCherry | Red Fluorescent Protein. From Chin-Sang plasmid. |
| YFP | Yellow Fluorescent Protein. From Chin-Sang plasmid. |
| eCFP | Enhanced Cyan Fluorescent Protein. From Chin-Sang plasmid. |
Backbones
- Since it is possible to microinject plasmids into C. elegans, we're working on a combination backbone that will produce GFP in both E. coli and C. elegans, as well as providing antibiotic selection markers for E. coli, and the cutsites from Standard 10.
- Another project we're pursuing is the possible usage of double-stranded RNA as a liaison for DNA import. If this works, we'll have hybrid strand backbones for that method of transformation as well.
Optogenetics (R.C. elegans)
Promoters
| Name | Description | Strength |
|---|
Coding Squences
| Name | Description |
|---|---|
| ChR2 | Channelrhodopsin-2. Permits entry of positive ions in response to 460 nm light. Can be used to trigger nerve cells. |
| NpHR | Halorhodopsin. Permits entry of chloride ions in response to 580 nm light. Can be used to block nerve cells from firing. |
