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Team:Stockholm/17 October 2010
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Contents |
Mimmi
clone non-CPP operon
colony PCR
| mix | x12 | primers | samples | |||
|---|---|---|---|---|---|---|
| mastermix | 24.5 | pEX_VF | pEX.SOD.RBS.yCCS 1-4 | |||
| DNA | 0.5 | pEX_VR | pEX.SOD.his.RBS.yCCS 1-4 | |||
| tot | 25µl | pEX.his.SOD.RBS.yCCS 1-4 | ||||
| positive control (pSB1C3.LMWP.SOD.his) | ||||||
Gel
| well | sample | well | sample |
|---|---|---|---|
| 1 | ladder | 12 | ladder |
| 2 | pEX.S_yC 1 | 13 | pEX.hS_yC 1 |
| 3 | pEX.S_yC 2 | 14 | pEX.hS_yC 2 |
| 4 | pEX.S_yC 3 | 15 | pEX.hS_yC 3 |
| 5 | pEX.S_yC 4 | 16 | pEX.hS_yC 4 |
| 6 | pEX.Sh_yC 1 | ||
| 7 | pC.Sh_yC 2 | ||
| 8 | pC.Sh_yC 3 | ||
| 9 | pC.Sh_yC 4 |
new colony PCR
| mix | x7 | primers | samples | |||
|---|---|---|---|---|---|---|
| mastermix | 24.5 | pEX_VF | pEX.SOD.his.RBS.yCCS 1-7 | |||
| DNA | 0.5 | pEX_VR | ||||
| tot | 25µl |
ON cultures
- pEX.SOD.RBS.yCCS 4
- pEX.his.SOD.RBS.yCCS 3
- pEX.SOD
- pEX.yCCS
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