Team:Caltech/Protocols
From 2010.igem.org
- Making V1012 competent
- Making stock solutions
- Freezer (glycerol) stocks
- Plating antibiotics
- Sequencing primers
- Making synthase casette
- Sources of sequences
- Optimizing & checking genes
- Chemical/Crosslinking reactions
- Digesting substrate (hydrolysis) + epoxidation (MCPBA)
- crosslinking (AIBN3) + UV initiation
- Inducing lysis via 632nm light initiation
- Making cell/glycerol/agar mixture
- Applying this to plates