Introduction

As previously described in the design of the switch, each half switch contains a nut site followed by a terminator, as well as an anti-terminator. The roles of these parts are to increase the stability of the current state and thereby also the robustness of the switch.

As the P_RM promoter is not very well repressed by the GogR/GtgR repressors, transcription is promoted even in their presence. In order for our switch to be robust, the spontaneous switching of states should be prevented. This is prevented by ensuring that the anti-repressor protein of the inactive half of the switch is not expressed by placing a terminator in front of the anti-repressor. The anti-terminator of the active state is expressed, allowing continued transcription past the terminator.

The regulatory systems utilized in our switch can be divided into:

• The Repressor - Anti-Repressor regulatory section
• The Terminator - Anti-Terminator regulatory section