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Team:Newcastle/1st April 2010
From 2010.igem.org
| Minutes for formal meeting 1st April 2010 |
| * Room: CBCB; Start: 4 pm |
| * Chair: Da, Minutes: Alan, Computer: Younus |
| 1. Roll call and apologies |
| Jen, Janetta, Steven, Younus, Da, Alan, Richard, Rachel, Jem, Matt |
| Phil, Harsh and Zoltan – Holiday |
| Apologies - Neil |
| 2. Approval of agenda and minutes |
| Approved |
| 3. Brief summary of previous meeting |
| 4. Research Feedback |
| 1. Nutrient
Using a glucose and starch concentration gradient. Uses the catabolic repression system to allow glue production. Have to test and find suitable starch and chemoattractant!!!!!!!! |
| 2. Sporulation, germination and secretion control
AbrB gene maybe a suitable gene to repress sporulation by overexpressing it. Can also activate antimicrobial peptides like TasA. Immune the concrete from future attack by concrete destroying bacteria? Need to find what other genes that are involve in the pathway of AbrB. |
| 3. Quorum Sensing
Number of competence cells not an issue. Sin operone to control a lot of pathway. |
| 4. Other points
A. FtsZ knockout to allow filamentous growth, thereby forming a mesh with glue either secreted out or remains in the cells until lysis. B. Is spider silk able to resist the alkali conditions? C. Is it possible to produce spider silk in Bacillus? |
| 5. Voigt paper feedback
Salmonella to secrete spider silk contained in SPI through T3SS. SPI to contain ADF1, ADF2 and ADF3 genes. Does Bacillus have a T3SS? Can we allow proteins to fold in the cells then induce lysis? To check out Bacillus secretion system. |
| 6. Visa/Passport update
Harsh to apply on May, June period |
| 7. Travel plans
Too early to book flights and hotels. May have other members joining. |
| 8. Tutorial
Next tutorial to be on the week after |
